FastQCFastQC Report
Mon 27 Feb 2023
SRR3126558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356271
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245091.0401604908773228No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32430.13763272560753834No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28900.12265142676712484No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG26180.11110776307139544RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77850.085.736081
CGTAGGG5500.080.32232
TAGGGCA15550.075.861874
ATAACGG1700.074.705851
AGGGATG37000.073.79975
AAGGGTA19500.073.5098344
AGGGTAC12900.072.1366045
ATAGCGG5650.071.5961761
TAAGGGA17350.071.512953
TAGGGTA11100.071.132254
TAGGGCG4300.071.0436254
AGAGGGC39550.070.586263
TAGACGG3600.070.555531
AGTAGGG38650.070.526042
ATAGGGC17000.070.497133
AGGGTAG12550.070.403515
ACGGGTA3350.070.14644
TAGGGAT16450.069.9971544
GAGGGTA12750.069.6677554
AGTAAGG15000.068.9876251
Produced by FastQC (version 0.11.5)