FastQCFastQC Report
Mon 27 Feb 2023
SRR3126557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464806
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200800.8146685783789881No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT59460.24123602425505292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69000.084.509111
ATAGGGC16000.074.609743
CGTAGGG6750.073.840632
ATAGGGA22400.073.434793
ACGGGAT5950.071.87974
AGTAGGG39950.071.208522
GAATAGG16200.070.885291
AATAGGG37050.070.563472
GTAGGGA19950.070.202923
TAGGGCA13550.070.0638354
TATAGGG21100.069.5289152
AGGGATG32500.069.412835
AGGGCAT21900.068.673275
GTAGGGC12700.068.091973
ATAGAGG16000.067.947471
TAGGGAC12850.067.662874
GGTAAGG16300.067.56311
AGAGGGC38300.067.245563
TAGGGTA7650.066.964854
AAGAGGG86500.066.645012
Produced by FastQC (version 0.11.5)