FastQCFastQC Report
Mon 27 Feb 2023
SRR3126556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519242
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201630.8003597907624594No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56740.22522647685295816No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG25660.10185603447386159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67400.083.034491
CGTAGGG8650.075.51362
GTAGGGC12400.073.1412053
AGTAGGG42800.072.6843262
ATAGGGA24150.071.8018653
TAGGGCA13050.071.298634
ATAAGGG33650.070.662982
ACGGGAT6700.070.137734
TAGGGCG4450.069.696424
ATAGGGC16750.068.734973
AGGGCTT27600.067.934495
TATAGGG23650.067.160222
CTAACGG1900.066.831561
TAGAGGG47000.066.7890242
AGGGACT43550.066.684795
GAGGGAT30550.066.450624
AGGGATG33000.066.3587955
ATAGCGG4850.065.938431
GGTAAGG16700.065.897791
ATGAGGG39150.065.777192
Produced by FastQC (version 0.11.5)