FastQCFastQC Report
Mon 27 Feb 2023
SRR3126554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614845
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239550.9161154867688142No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51870.1983673984500037No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG26810.10252997787631771TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76650.083.558811
AGTAGGG38550.072.46042
ATAGGGC15900.071.826053
ATAGAGG18150.071.0428161
AGTACGG4150.070.305591
GTAGGGC13200.070.13993
ACGGGTA2500.069.555994
ATGCGGG14300.067.414992
CGTAGGG7400.067.361672
GAATAGG15300.067.3595051
GGTAAGG15750.067.22771
AGGGATG33750.067.11915
GAGGGAT29850.066.599184
AGGGACT36200.066.211665
TGTAGGG18500.066.090692
AGAGGGC40200.065.702683
AGGGCAT23250.065.6951455
ATAAGGG34000.065.283252
AATAGGG38750.065.169032
AAGAGGG87700.065.096522
Produced by FastQC (version 0.11.5)