FastQCFastQC Report
Mon 27 Feb 2023
SRR3126552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047568
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308241.0114294414431442No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56850.18654218708163361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97500.084.6868441
GGTAAGG16800.073.0511861
ATAGGGC19400.072.434353
AGTAGGG45150.071.6152952
ACGGGAT7350.070.9758764
ACGGGTA3050.070.881534
CGTAGGG7150.069.6746752
GTAGGGC15700.069.149253
GTACGGG7900.069.009052
CGATACG551.6734703E-1068.357819
ATAACGG2000.068.181111
GTAGGGT11250.068.0942153
ATAGCGG7400.067.9904861
ATAGAGG23050.067.931211
TAGAGGG57600.067.640562
TAGGGCA15950.066.886784
AGGGCAT25000.066.5485155
TAGCGGG18350.066.3344042
TAGACGG4750.066.3250051
AGGGTAG13400.066.2875755
Produced by FastQC (version 0.11.5)