FastQCFastQC Report
Mon 27 Feb 2023
SRR3126551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610267
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138670.861161534080994No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34880.21661004044670856No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG25630.15916615070668405No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC23460.14569012468118642No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG22120.13736852335668556No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTACTCGG20530.12749438447164352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48200.082.38771
CGTAGGG6150.080.263272
TAGGGCA8150.076.691694
ACGGGTA1500.075.192454
ACGGGAT4000.074.017564
GTACGGG4000.072.867582
TAGGGAT11750.070.392934
GTAGGGA14500.069.358553
AGGGACT28400.069.334595
ATAGGGC11150.068.701623
GGTAAGG10150.068.142531
TAGGGTC3450.068.10914
TAAGGGA12500.068.0491643
AGTAGGG24250.068.045282
ATAGGGA15200.068.0194853
ATAAGGG22100.067.8579942
GAATAGG10150.067.678981
AGAGGGC23550.067.649263
AGGGAAT19600.067.3758855
GCGTAGG3550.066.268731
Produced by FastQC (version 0.11.5)