FastQCFastQC Report
Mon 27 Feb 2023
SRR3126550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637574
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138600.8463739653902662No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34640.21153242540489772No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG27890.17031291410342372No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC25730.15712267048695203No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG24250.1480849109719622No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTACTCGG19180.11712447803885503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47650.082.13621
AGTAGGG27250.074.4927142
ACGGGAT4400.073.6896064
CGTAGGG4700.072.982972
GTAGGGA14400.072.443643
GGTAAGG9450.071.681131
TAAGGGA13200.071.553683
AGATAGG9550.071.423121
CGGGTAT1750.069.8144155
TAGGGCA8350.069.7822654
AGGGAAT19350.069.6964655
AGGGACT25650.069.432325
ATAGCGG3850.068.4229051
TAGGGTC4750.068.259854
GTAGGGC8350.068.093983
ATAAGGG22850.068.0672762
ATAGGGC10300.067.5202943
AAGGGGC46850.067.401494
ATAGGGA14100.067.319693
TAGAAGG11900.067.201061
Produced by FastQC (version 0.11.5)