FastQCFastQC Report
Mon 27 Feb 2023
SRR3126549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838968
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175590.9548290127941323No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38740.21066163195879428No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG30130.16384189393181392No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG27770.15100860917645115No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC26460.1438850485707201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60800.082.45931
TAGGGCA10200.075.104564
CGTAGGG5200.075.015952
TAAGGGA13250.073.0685043
AGTAGGG28750.071.7638242
ATAGGGA16750.071.2685243
GAATAGG12150.071.22421
ATAGCGG4500.071.069371
ATAGGGC11600.070.091763
TAGAGGG33050.069.110442
GTAGGGA14950.068.846493
TAGGGAT12800.068.293894
TAGACGG2550.068.241351
GGTAAGG10900.068.173661
AGATAGG10900.068.173661
AGGGATG25250.067.3792345
AGGGACT30000.067.363725
ACTAGGG7050.067.33042
CGGGTAT1900.066.786555
TAGGGTC4100.066.48494
Produced by FastQC (version 0.11.5)