FastQCFastQC Report
Mon 27 Feb 2023
SRR3126548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847909
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138360.7487381683838328No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32890.1779849548868478No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29250.1582870152155761No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG21440.11602302927254535No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACTTCCG18540.10032961579818055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53600.082.7103651
AGGGATG37250.077.84335
CGTAGGG6250.077.49582
ATAGCGG4500.077.3914641
AGGGTAC8150.076.116485
ACGGGAT7500.075.1938554
GAGGGAT28300.074.2306754
AAGGGTA11800.072.883874
AGGGCAT18350.071.966875
GTAGGGC10050.071.546393
TAGGGCA10650.071.4871144
AAGGGAT40550.071.392444
ATAGGGC10750.071.259293
GTAGGGA17250.071.107233
AGTAGGG32600.070.968962
ATAGGGA20550.070.665763
ACGGGTA2100.069.375284
GGATGGC30650.069.3059167
TAGCGGG11450.068.996142
TAGACGG1850.068.685631
Produced by FastQC (version 0.11.5)