FastQCFastQC Report
Mon 27 Feb 2023
SRR3126547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893209
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139010.7342559643441374No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31890.16844416015347488No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31880.1683913397834048No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG22500.11884583265767276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5450.083.632782
ACGGGTA1700.082.922744
CGTTTTT51500.082.298031
AGATAGG10150.076.006311
GTAGGGC9550.075.7737353
ACGGGAT6150.075.641724
AGGGATG39750.075.30155
GAATAGG12850.074.679071
ATAGCGG5000.074.323981
AGTAAGG11000.073.554221
ATAGGGA22750.072.911273
TAGCGGG10900.072.424272
TAGGGCA9800.071.922794
ATAGGGC12800.071.5856553
AGGGAAT23050.071.5545965
AGGGTAC8750.071.4241265
TGTAGGG13100.071.381082
AGTAGGG31250.070.973022
AAGGGAT42100.070.651764
GAGGGAT29500.069.767554
Produced by FastQC (version 0.11.5)