FastQCFastQC Report
Mon 27 Feb 2023
SRR3126546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125008
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173970.8186792708545098No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37070.1744464020841333No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36660.17251699758306793No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG24060.11322310316008223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59850.082.589081
AGGGATG44200.076.0276265
ATAGGGC12850.075.710273
CGTAGGG5550.074.520772
AGGGTAC9300.073.783215
ACGGGTA2300.073.5634844
TAGGGCA11950.072.36654
ATAGGGA25850.071.271053
AGGGCAT18100.070.887835
GAATAGG14200.070.5464861
AAGGGAT46700.070.448024
AGATAGG10750.070.437111
GAGGGAT35050.070.397774
ATAGCGG6100.070.160981
AGTAGGG35450.070.133752
ACGGGAT7800.069.895794
CTACGAC9300.069.7402955
AAGGGTA14000.069.155524
ACTAGGG9900.068.836762
GATAGGG31050.068.5684362
Produced by FastQC (version 0.11.5)