FastQCFastQC Report
Mon 27 Feb 2023
SRR3126545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441211
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119870.8317310928101438No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46900.3254207746124613No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG28510.1978197501961892No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG27280.1892852607980372No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25850.17936304954652718No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGAGTTAC25710.17839164424917656No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC24710.17145303498238634TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42500.085.72031
AGGGATG30050.079.1360245
TAGGGCG2550.077.406484
GCTACGA8200.077.3727654
CGCTACG8350.075.982843
CTACGAC8400.075.5305565
GGATGGC22850.075.482817
GGTAAGG8500.075.309821
TAGGGCA9000.075.194864
ACGGGAT5450.074.160074
ATAGCGG2750.073.5982361
AGTAAGG9550.073.436941
ATAGAGG10150.073.269411
AGAGGGC20550.073.182353
GAGGGAT24600.072.787714
TCCGCTA8800.072.742451
AGGGCAT14050.072.25135
CGAGGGA10050.072.0149843
AGTAGGG21200.071.847722
GTACGGG2700.071.3877942
Produced by FastQC (version 0.11.5)