FastQCFastQC Report
Mon 27 Feb 2023
SRR3126543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628053
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155320.9540229955658692No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54650.3356770326273162No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG36340.2232114065082648No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG34190.21000544822557987No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC30370.18654183862564672TruSeq Adapter, Index 27 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27950.17167745767490372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47200.085.788661
ATAGCGG3200.082.301311
CTACGAC9850.078.7268455
AGGGATG34150.077.893445
CGTAGGG4700.076.995982
AGGGATC18350.076.57915
TCCGCTA10150.076.45161
GAGGGAT28050.075.2295764
TACGACC10400.074.56346
TAGGGCA9350.073.889184
ATAGGGA18500.073.417783
AGTAAGG10650.072.862321
GTAGGGC7950.072.713183
TAGAGGG28600.072.303922
GTACGGG3250.072.303922
TAGCGGG9300.072.2650452
GGATGGC26450.071.606847
AGTAGGG24350.071.606122
ATAAGGG21800.071.358662
CGCTACG10900.071.1430743
Produced by FastQC (version 0.11.5)