FastQCFastQC Report
Mon 27 Feb 2023
SRR3126542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946067
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83880.8866179668036196No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63150.6675002933196064No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGCTAGT37820.3997602706785037No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC36130.3818968424012253Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG27800.29384811012327877No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG26150.27640748488214895No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC14620.15453450971231425No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG13820.1460784489893422No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG13200.13952500192903886No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT11870.1254668009770978No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT11460.12113306985657463No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT11080.11711644101316292No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10520.11119719850708248No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCGCTAGTTC9800.10358674385640763No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT9620.10168413019373893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33900.086.493061
TAGGGCG2200.083.3133544
AATGCGG2550.083.0548861
GGGCGAT3300.082.601276
CGTAGGG2850.082.499322
AGGGATG28150.082.47485
AAGGGCG3100.081.866234
GGATGGC23800.081.55417
ACGGGAT5100.081.093344
CACGGGT353.8655344E-880.5667653
GGATGAC12100.080.011897
TAGCGGG4200.079.4939962
TAACGGG3200.079.354042
CGAGGGA5050.079.1043243
CGAAGGG8050.078.861162
CGCTACG12300.078.3288043
TACGGGA2100.078.328793
GATGGCA23350.078.295258
GATGACC11600.078.193758
TCCGCTA12550.077.62821
Produced by FastQC (version 0.11.5)