FastQCFastQC Report
Mon 27 Feb 2023
SRR3126541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970211
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85430.8805301114912117No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66110.6813981700887745No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC36820.3795050767307318Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGCTAGT36420.37538226220894216No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG29650.3056036264276534No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG27430.28272200583172113No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC14710.1516165040388122No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG14390.1483182524213805No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG13250.13656823103428017No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT12550.12935330562114838No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT12150.1252304910993588No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT11200.11543880661010852No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT11150.11492345479488482No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA9880.10183351868820287No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT9760.100596674331666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG2400.086.202821
CGTTTTT35350.085.925721
AGGGATG26900.082.281555
GGATGGC23100.081.983587
CGGGAAT3600.080.932515
CGAAGGG7050.080.654772
CTAACGG353.8528015E-880.605241
GTACGGG1750.080.559552
CGTAGGG2400.080.279822
AGGGCGA4450.080.257835
GATGGCA22200.079.803548
TGCGAGG1600.079.345791
TAGCGGG4800.079.30082
GTAGGGC4900.078.6414643
AGGGAAT17500.078.142765
GCGGGAT6200.078.069134
TAGGGTC2650.078.026224
GGATGAC12050.077.9967967
GATGACC11700.077.920138
AGGGATC10850.077.527735
Produced by FastQC (version 0.11.5)