FastQCFastQC Report
Mon 27 Feb 2023
SRR3126540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080741
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111691.0334575999244964No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC76800.7106235444014801No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC38140.35290601540979755Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG32570.30136730261922146No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG32520.30090465708250175No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC17650.16331387446205892No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGCTAGT16080.1487868046090599No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG14840.137313195298411No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT14550.13462985118543666No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT13540.12528441134369844No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT12700.11751196632680724No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT12590.11649414614602388No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT10910.10094925611224151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46450.086.1580661
TAGCGGG5650.083.1844942
GTACGGG2150.083.068422
CTAACGG800.082.298161
AGGGATG31650.082.118725
ATAGCGG2700.081.8627241
GATGGCA24650.081.0331658
TAACGGG3150.080.570122
GCGGGAT6300.080.570124
GACCGAT353.8648068E-880.5701148
CGAGGGA6650.080.5701143
GGATGGC26800.080.494967
CGTAGGG3350.079.968852
AGGGAAT20400.079.714395
AATGCGG2600.079.5850451
TCCGCTA15500.079.188281
GGATGAC14300.078.879847
GGTAAGG5500.078.664221
GAGGGTA6100.078.588894
CGAAGGG8700.078.332062
Produced by FastQC (version 0.11.5)