FastQCFastQC Report
Mon 27 Feb 2023
SRR3126539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047713
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.31000856150491596No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG25590.24424627736794333No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG16790.16025380996513358No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC16780.16015836397944858No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG15990.1526181311103327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT32500.085.886971
ATAGCGG2100.085.176591
TAGCGGG4650.082.936042
CGTAGGG2650.081.638382
CGAAGGG7350.081.264112
ACGGGAT3150.077.578874
AGCGGGT2800.077.20593
TAGACGG1100.077.025721
GGGCGAT4500.075.191836
AACGGGC2700.074.843723
AGTACGG1150.073.676771
GTAGGGT4350.073.463283
TACGGGA2500.073.3120353
TAAGCGG1800.073.221981
CTAGCGG900.073.221981
AGGGATC9150.072.4183655
GTACGGG2600.072.355092
ACGCGGG2350.072.047192
GCGGGTA1900.071.729044
CGAGGGA5950.071.084713
Produced by FastQC (version 0.11.5)