FastQCFastQC Report
Mon 27 Feb 2023
SRR3126537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215476
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42630.350726793453758No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG20630.16972774452148787No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG19860.16339277780885844No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC17920.14743195258483097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG3800.085.336282
CGTTTTT42800.085.1799241
TCGTAGG750.081.538251
ATCGCGG1100.081.253161
CGAAGGG8600.080.8782042
GTACGGG1850.078.751372
CGTTCGG301.4567831E-678.327999
ACGGGTC1500.078.327994
ATAGCGG2700.076.659891
AAGGGCA20800.076.143844
AGCGGGT4300.075.413463
AGGGCGC1700.074.641965
TAGCGGG6950.074.383412
AGGGTAC6650.074.205465
AGGGCAT10750.073.883325
AGGGCTC10850.073.635525
GGGCGAT5750.073.560196
CGGGTAT1600.073.432495
AGTACGG1100.072.7001951
AAGGGCG5050.072.58914
Produced by FastQC (version 0.11.5)