FastQCFastQC Report
Mon 27 Feb 2023
SRR3126536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915722
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92581.0110055235104105No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT44790.48912224452399305No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG35270.3851605618299003No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG31870.3480313894391529No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC31610.34519209978574283No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27710.3026027549845914No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATG16980.1854274550573209No Hit
GAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT14860.16227632403720782No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT12250.13377422405489875No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12250.13377422405489875No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT11830.12918767923015936No Hit
AGAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT10770.11761211372010283No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT10420.11378999303282No Hit
AAAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT10100.11029548269016141No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9740.10636415855467052No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG9620.10505371717617355No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTAAGTCCTTC9470.10341566545305234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT35800.086.980491
CGGCATA500.084.598675
AAGGGCG2600.083.152544
CGTAGGG2100.080.592162
ACGGGAT2800.080.570174
AGGGCTC6050.079.238425
AGTAAGG4600.077.714611
AGGGATG17650.076.95635
TAGGGCG1650.076.907884
TAAGGGA9950.076.5214163
ATAGAGG5150.075.808481
ATAGCGG1800.075.783111
TAGCGGG4250.075.219352
GGCGTAT255.559208E-575.198827
CTACGAC6000.075.198825
TAACGGG2450.074.835582
GTAGGGA6900.074.245213
AGGGAAT15650.073.877435
AGTAGGG13100.073.568552
GTAGGGC4350.073.4701163
Produced by FastQC (version 0.11.5)