FastQCFastQC Report
Mon 27 Feb 2023
SRR3126535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947109
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92260.9741223027127818No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTAAGTCCT40920.4320516434750382No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG38820.4098789051735333No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC36930.389923440702179No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG35320.37292434133769187No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28910.30524469728405074No Hit
GAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT16150.17051891598538288No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATG15330.16186098960098572No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT13070.13799889980984237No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT12460.1315582472555957No Hit
AGAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT11420.12057746257294567No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG11000.1161429149126447No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10810.114136810018699No Hit
AAAGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT10030.10590122150671147No Hit
GATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC9970.10526771469809705No Hit
AAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT9860.10410628554897061No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT9820.103683947676561No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTAAGTCCTTC9620.10157225831451291No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9530.10062199810159127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT35350.084.232551
CGGGTAT1200.082.2414555
TACGGGT401.036824E-982.2414553
CGTAGGG2800.080.55882
GGATACG1000.079.891698
ACGGGAT2750.078.6100164
ACGGGTA850.077.403724
AGGGAAT17500.076.2663655
AGGGATG15900.076.256235
AAGGGTA5650.075.6912464
AGGGTAC3450.074.919755
TAAGGGA9550.074.798513
AGTAGGG13150.073.615842
TAGGGCG1600.073.429874
GGTAAGG4550.073.4027941
GTAGGGA8150.073.231653
ATAGCGG2900.072.992831
GGGCGAT2450.072.890386
ATAGGGA8650.072.801693
AGAGGGC11450.072.647473
Produced by FastQC (version 0.11.5)