FastQCFastQC Report
Mon 27 Feb 2023
SRR3126533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324766
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243271.0464278985497895No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46730.20100947794315643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86900.084.638231
ACGGGTA2400.074.411314
ATAGGGC13150.069.6907963
ATAGCGG5000.069.644851
ACGGGAT6200.068.97894
AGTAGGG40100.068.825142
TAGGGCA10450.068.808434
CGTAGGG7400.068.619162
AGTAAGG14550.068.5646441
ATAGAGG16900.068.4976651
TAGCGGG14800.068.301482
AGGGATG32600.068.0441745
ATAGGGA21300.067.957543
TAGGGTA7750.067.311284
AATAGGG33300.067.066062
GATAGGG34550.066.8169862
AAGAGGG77300.066.723772
GTACGGG5850.066.707652
GAATAGG14650.066.490571
ATAAGGG35750.066.152362
Produced by FastQC (version 0.11.5)