FastQCFastQC Report
Mon 27 Feb 2023
SRR3126532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356407
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246121.044471519563471No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45840.19453345708105602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82350.084.476471
TAGGGTA7000.073.167314
CGTAGGG7300.072.735132
AGTAAGG15550.070.780991
GTACGGG5600.070.482282
GAATAGG14700.070.074171
ATAGGGC13100.069.9442443
ATAGCGG5450.069.9068451
AGTAGGG37850.069.5201642
AGGGATG32250.069.3530965
GGTAAGG14650.069.3501361
TAAGGGA17900.069.0385063
TAGGGCA10900.068.973484
ATAGGGA20700.068.325813
ATAAGGG32600.067.167152
AGACGGG16750.067.045822
GTAGGGA17400.066.971663
TATAGGG20350.066.730142
GTAGGGC9950.066.114033
TAGCGGG14600.065.9765552
Produced by FastQC (version 0.11.5)