FastQCFastQC Report
Mon 27 Feb 2023
SRR3126531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637601
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304471.1543444213131555No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53690.20355618609486423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99800.085.256841
CGTAGGG9450.076.0898362
TAGGGCA13300.073.145184
ATAGCGG6950.073.090361
AGGGATG36350.071.36775
AGTAGGG47700.071.135412
ATAGGGA22150.071.0784763
TATAGGG22700.070.598512
TAGGGTA8600.070.4949954
ATAGGGC15950.070.421333
TAGCGGG17200.069.4020542
TAAGGGA22700.069.356313
GTAGGGT11500.069.064673
GTAGGGA21350.068.8990863
GAATAGG16050.068.8674551
ATAAGGG38300.068.2249152
TAGGGAT16600.067.946984
GGTAAGG15800.067.87331
AGTAAGG17150.067.467121
TAGGGAC13950.067.041834
Produced by FastQC (version 0.11.5)