FastQCFastQC Report
Mon 27 Feb 2023
SRR3126530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580981
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265881.0301509387322108No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39510.15308132837862812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90700.084.335591
ATAGGGC17200.072.677883
AGTAGGG39550.071.457262
AGATAGG14650.071.0099951
ATAGCGG6300.068.740411
TAGGGCA15900.068.275354
CGTAGGG8150.068.083642
GTAGGGC13950.067.3759463
TATAGGG22800.067.2358862
ATAAGGG33700.066.5590062
ATAGGGA23250.066.297933
AAGAGGG89900.066.2728042
AGGGATG36550.065.702615
GAATAGG15450.065.505051
ATGAGGG38150.065.081532
TAAGGGA21550.064.985743
ATAGGGT8550.064.858213
GGTAAGG16800.064.724331
TAGAGGG47500.064.645452
AGGGCAT22600.064.461795
Produced by FastQC (version 0.11.5)