FastQCFastQC Report
Mon 27 Feb 2023
SRR3126529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610032
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267481.0248150214250247No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39350.15076443507206044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93700.084.348531
ATAGGGC17050.079.10113
TAGGGCA15900.075.660264
ATAGAGG18750.070.754761
GAATAGG17050.069.80781
TATAGGG20950.069.5348742
AGTAGGG42000.069.257432
ATAGCGG6250.069.249341
ACGGGAT5350.068.511874
GTAGGGC14700.067.131593
AATAGGG39450.066.944412
ATAGGGA20250.066.833233
AGGGATG31800.066.4982765
TAGACGG3200.066.156171
AAGAGGG90600.065.9756852
TAAGGGA20300.065.974163
GGATAGG16150.065.250421
AGAGGGC38800.065.159183
TAGAGGG47600.064.7622452
AGATAGG16200.064.758641
Produced by FastQC (version 0.11.5)