FastQCFastQC Report
Mon 27 Feb 2023
SRR3126528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919677
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335531.149202463149177No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45320.1552226496287089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114700.084.222131
ATAGGGC18850.075.046753
AGTAGGG46350.071.8907852
ATAGCGG8050.071.277621
ATAGGGA26350.070.095263
CGTAGGG9350.069.868262
TAGGGTA9400.069.496624
GAATAGG18350.069.458031
ACGGGAT6500.069.412014
TAGAGGG52150.068.8518752
TAGGGCA15000.067.990034
TATAGGG25750.067.1656652
GTACGGG6100.067.0295262
ATAGAGG22700.066.921531
AGAGGGC42150.066.566163
ATAAGGG42200.066.487292
AAGAGGG102200.066.173882
AGGGATG38350.065.93165
AAGGGAC26650.065.779164
AGTAAGG18900.065.6948551
Produced by FastQC (version 0.11.5)