FastQCFastQC Report
Mon 27 Feb 2023
SRR3126527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201405
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262511.1924657207556084No Hit
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT28040.12737320029708302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168200.089.8746641
ACGGGAT10800.080.064534
ATAGGGC18050.075.7638243
ATAGCGG4100.075.7613751
AGGGATC29050.074.899865
CGAGGGA16350.073.868883
TAGGGCG4650.073.776154
TAGGGCA19450.073.2097854
CGAAGGG18350.073.035032
AAGGGAT63250.072.5162664
AGGGATG46800.071.6965945
ATAACGG1850.071.231831
TAAGGGA30250.070.841153
GTACGGG4250.070.813072
CGTAGGG6700.069.483672
AGGGCGA11800.069.296835
GAGGGAT51900.069.178664
AGGGATT48000.069.0230265
AGAGGGC41000.068.543053
AAGGGCG12450.068.321184
Produced by FastQC (version 0.11.5)