FastQCFastQC Report
Mon 27 Feb 2023
SRR3126526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227934
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265411.1912830451889507No Hit
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT28970.1300307818813304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT167600.090.0102841
ATAGCGG3700.077.552411
ACGGGAT10500.076.979894
AGGGATG46000.074.781025
AGGGATC28500.074.365215
AAGGGAT62550.073.927484
TAGGGCA20700.072.192974
CGTAGGG5800.072.110772
CGAGGGA16050.072.027473
AGCGGGA19750.071.620523
GTAGGGC13000.071.213283
TAGGGAT27200.071.008624
ATAGGGC19300.070.855543
TAGCGGG11600.070.49032
CGAAGGG19350.070.429592
GTACGGG5000.069.550442
GCGGGAT14400.069.511284
AAGCGGG23650.069.3477552
ATGCGGG11400.069.253632
TAAGGGA30800.069.117123
Produced by FastQC (version 0.11.5)