FastQCFastQC Report
Mon 27 Feb 2023
SRR3126525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528496
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326311.2905300225905045No Hit
CGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT32210.12738798083920244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT199800.089.204181
ACGGGAT13150.078.988114
GTACGGG5400.076.590612
GCGGGAT15100.075.012794
ATAGCGG5050.074.5168841
CGTAGGG8200.074.510272
CGAGGGA17250.073.8372343
TAGGGCG5550.073.6752244
AGGGATG53550.073.461745
AGGGATC34850.073.095695
AAGGGAT74100.073.004954
TGTACGG1750.072.574121
TAGGGAT30600.072.4962844
CGAAGGG22050.072.043492
ATAGGGC20050.071.730233
TAGGGCA22150.070.446524
GTAGGGC15950.070.131233
TAAGGGA35900.069.910433
AAGGGCG13500.069.62924
TAGCGGG12500.069.5582052
Produced by FastQC (version 0.11.5)