FastQCFastQC Report
Mon 27 Feb 2023
SRR3126524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770143
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152100.8592526140543448No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32570.18399643418639058No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG32340.182697104132265No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27860.15738841438234086No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC25410.1435477246753511No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCACCCG24460.13818092662570197No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG23030.1301024832457039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48950.083.654081
CGTAGGG4350.076.742392
TAGGGCA10200.074.181514
ATAGGGC12550.074.146633
GTAGGGT8750.073.583843
ATAGCGG3800.073.07841
GAATAGG12200.070.986961
AATAGGG24150.070.868062
GGTAAGG10500.070.8252941
ATAGGGA13650.070.581543
AGGGCGA11500.070.291145
AGTAGGG29700.069.973252
AGGGAAT21700.069.737455
AGGGACT28850.069.721685
AGGGATG25200.069.562995
AGGGCAT16400.069.34925
ACGGGTA1900.069.2587054
TAGGGCG4100.068.776064
ACGGGAT3350.068.741854
TAGGGTC6550.068.163554
Produced by FastQC (version 0.11.5)