FastQCFastQC Report
Mon 27 Feb 2023
SRR3126523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841347
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154720.8402544441650596No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG35220.19127301915391287No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32150.17460044195906585No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30450.1653680702225056No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC26560.1442422313664942No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG24220.1315341432114642No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCACCCG22360.12143284237028654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50050.085.0570451
AGGGCGA12550.072.2694245
TAGACGG2550.071.9432751
AGGGATG24250.071.120595
CGTAGGG5100.070.949572
TAGGGCA11150.070.806884
AGTAGGG29500.070.5685042
AGAGGGC25950.069.358943
ATGAGGG27050.068.9687652
AGGGCTC16250.068.249535
AGGGACT32450.068.209875
ATAGAGG11400.068.083981
AGGGTAC8700.068.060055
GTAGGGT9950.068.01123
AGGGAAT22050.067.560325
AAGAGGG63650.067.111152
AAGGGAC16400.067.05224
ACGGGAT3650.066.950144
AAGGGCG9300.066.700954
AAGGGTA13600.066.6898044
Produced by FastQC (version 0.11.5)