FastQCFastQC Report
Mon 27 Feb 2023
SRR3126522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047267
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191740.9365656751171196No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36310.1773584002477449No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG35880.17525803913216986No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34300.167540433172615No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC28590.1396495913820718No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG27700.13530233232890485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62350.083.201931
CGTAGGG4850.077.5218662
TAGGGCA11750.073.996284
ACGGGAT4700.072.996324
GGTAAGG10800.072.725551
AGGGAAT24400.071.459515
AGGGATG27300.069.893915
ATGAGGG31250.069.782082
TAGGGCG5000.069.5564964
AGTAGGG33450.069.267062
GTAGGGC10400.069.140753
GAGGGAT27350.068.7351154
AGGGCGA13850.068.545295
AGGGACT33350.068.4883045
AATAGGG29000.068.22762
AGAGGGC27900.068.222373
AAGGGCG10100.067.937174
CTACGAC7450.067.4999545
ATAGGGC13200.066.936023
ATAGGGA18050.066.91633
Produced by FastQC (version 0.11.5)