FastQCFastQC Report
Mon 27 Feb 2023
SRR3126521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169034
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330871.5254256042090626No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26750.12332678971376197No Hit
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT24620.11350675000945121TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117550.087.831891
AGGGTAC15150.078.172695
TAGGGTA10550.074.838324
CGTAGGG5650.073.2355042
ATAGGGC14950.072.9313053
AGTAGGG36600.071.943972
ACGGGTA3350.071.547184
TAGGGCA11550.070.800194
ATAACGG1850.068.679991
AAGGGTA19400.068.5570144
GAATAGG15400.068.4487461
AGGGCAT17700.067.97265
TAGGGCG3600.067.8840944
GAGGGTA15750.067.734894
GTACGGG5300.067.425662
ATAGGGA17400.067.2538763
GTAGGGC9300.067.210313
GTAGGGA17350.067.176813
TAAGAGG15050.066.913771
GGTAAGG12050.066.780131
Produced by FastQC (version 0.11.5)