FastQCFastQC Report
Mon 27 Feb 2023
SRR3126520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191628
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334271.525213220491799No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27020.12328734621021452No Hit
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT24560.11206281357967685TruSeq Adapter, Index 16 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT22270.10161396003336332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116100.087.9182051
ATAGGGC13650.079.1790543
AGGGTAC16800.076.080745
TAGGGTA9750.075.667634
ACGGGTA3500.072.5004654
TAGGGCA10900.072.426574
AAGGGTA19500.072.2939154
AGTAAGG12300.071.1310351
ATAGGGA18400.070.741953
AGGGCAT18450.069.7861565
GTACGAT1700.069.106058
AGTAGGG36400.069.064932
GATAGGG31400.068.689162
GGTACGA3900.068.6807867
ATGCGGG12950.068.579962
CGTAGGG6650.067.835152
GAGGGTA15850.067.299484
GAATAGG14400.067.290851
ACGGGAT5600.067.1300664
ATAAGGG29450.067.014562
Produced by FastQC (version 0.11.5)