FastQCFastQC Report
Mon 27 Feb 2023
SRR3126519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447775
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414331.6926800870178018No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31030.12676818743552817No Hit
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT25930.10593293909775205TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT136650.088.449471
AGGGTAC20350.076.212885
TAGGGTA11050.074.431144
ACGGGTA3550.074.13764
ATAGGGC16100.073.562013
ATAGGGA20350.072.2867663
AAGGGTA22100.072.091874
TAGGGCA13150.071.8371664
AGAGGGC35800.069.97183
AGTAGGG39700.069.372292
GAGGGTA17750.068.5772864
GTAGGGA18100.068.289833
TAGAGGG48400.068.263552
GAATAGG16750.067.947321
ATAAGGG34950.067.504922
TAGGGTC7450.067.5004044
ATGCGGG11050.067.200682
AGTAAGG16900.066.509391
ACGGGAT7850.066.455694
CGTAGGG7150.066.38872
Produced by FastQC (version 0.11.5)