FastQCFastQC Report
Mon 27 Feb 2023
SRR3126518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116074
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213101.0070536285593037No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36350.17178038197151896No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC23010.10873910836766579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69600.084.930111
TAGGGCA9100.071.269354
CGTAGGG5600.070.5280152
AGTAGGG35150.070.360812
GTAGGGC9750.068.928133
AGAGGGC29850.068.802183
GTAGGGA15850.068.7897643
ATAAGGG26800.068.773582
ATAGCGG4700.068.091411
ATAGGGA16850.067.496393
AGGGATG31100.067.396845
GAATAGG12350.067.0697861
AAGAGGG73700.066.795862
TAGTAGG11400.066.466421
ATAGGGC11550.066.3243
GAGGGAT25700.066.197344
AGTAAGG11900.066.0466461
AGGGACT30700.065.978745
AGGGTAC8700.065.3628855
TAGACGG3400.065.057921
Produced by FastQC (version 0.11.5)