FastQCFastQC Report
Mon 27 Feb 2023
SRR3126517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208498
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216280.9793081089500647No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36360.16463678029140166No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC22960.10396205928191919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72600.084.36761
TAGGGCA10400.075.012454
AGGGCAT19400.071.947115
GTAGGGC10150.070.840893
CGTAGGG6450.070.674192
AGTAAGG12000.070.17351
ATAAGGG27900.070.070942
AGTAGGG35000.069.015062
GTAGGGT9300.068.219643
AAGAGGG78850.067.169332
AGGGATG29250.066.999085
ATAGAGG14900.066.934641
AGATAGG13850.066.914121
GAATAGG12450.066.8813861
GAGGGTA11750.065.594084
TAGAGGG41100.065.518152
ATAGGGC12000.065.402423
GTACGGG4900.065.2171252
AGAGGGC34150.064.5417563
AAGGGGC64050.064.275224
Produced by FastQC (version 0.11.5)