FastQCFastQC Report
Mon 27 Feb 2023
SRR3126516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516714
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271301.0779929701984412No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42380.16839418384448926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87900.085.696911
TAGGGCA11800.074.480744
AGTAGGG40050.070.057752
ACGGGTA3500.069.826414
CGTAGGG6400.069.763462
AATGCGG6150.068.811161
AAGAGGG93400.068.6862952
ATAGGGA22350.068.132113
GTAGGGC12350.068.119353
AGGGCAT24800.067.655165
AGGGAAT26550.067.444235
TAGAGGG46150.067.111672
TATAGGG19550.067.072082
AGTAAGG18000.066.874261
ATAGGGC14250.066.622493
ATAAGGG32850.066.527622
AGGGTAC10900.066.40165
AGGGATG31950.066.195125
ATAGAGG17500.065.8293461
GAATAGG16250.065.684661
Produced by FastQC (version 0.11.5)