FastQCFastQC Report
Mon 27 Feb 2023
SRR3126515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451670
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216560.8833162701342351No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24560.10017661430779835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84250.083.780781
ACGGGAT7200.075.716444
TAGGGCA13950.074.7899864
ATAGGGC14650.074.745153
ATAGCGG5400.073.199581
CGAAGGG22750.070.061262
TAAGGGA26150.069.3713763
AGGGATG40150.069.177785
AGTAGGG41900.069.0113752
CGTAGGG6450.068.521642
AGAGGGC36700.067.9975363
TATAGGG20500.067.8886952
AAGGGAT45050.067.8083654
GTAGGGC12850.067.66023
ATAGGGA23450.067.338143
ATGCGGG14050.067.263482
AGGGATC20400.067.0389945
TAGGGCG5400.067.0134054
TAGGGAT19700.066.7969364
TAGCGGG14800.066.714032
Produced by FastQC (version 0.11.5)