FastQCFastQC Report
Mon 27 Feb 2023
SRR3126514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502930
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217090.8673434734491177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81350.084.365271
ACGGGAT7650.076.169924
CGTAGGG7850.071.833372
ATGCGGG13700.071.343992
ATAGGGA23700.071.181873
ATAGCGG4650.070.812691
AGTAGGG41250.069.603622
ATAGGGC16550.069.5650943
TATAGGG22100.068.679142
AGGGATG37600.067.988335
CGAAGGG25500.067.8144452
GAGGGAT35500.067.774284
AGGGCAT26900.067.605495
AGGGATC21250.067.4472355
AGGGTAC8850.066.903765
AAGAGGG81550.066.841882
GGGCGAT9150.066.764496
TAGCGGG12800.066.44822
TAGGGAT18700.066.341554
AGTAAGG18550.066.1854551
Produced by FastQC (version 0.11.5)