FastQCFastQC Report
Mon 27 Feb 2023
SRR3126513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821759
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274470.9726911476139528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98650.084.956431
CGTAGGG8200.076.802672
ATAGCGG7250.072.655031
ATAGGGC18350.070.690083
TAGGGCA15700.070.647664
ACGGGAT9200.069.987124
AGGGATG41950.069.90985
TAGCGGG16750.069.586072
AGTAGGG46250.069.5071952
AAGGGAT53100.069.126084
ATGCGGG17750.069.107882
TAAGGGA28200.068.3313753
CGAAGGG29900.068.218782
GGTAAGG16800.068.027211
TATAGGG23250.067.920632
AGGGCAT28950.067.8598865
ATAGGGA28050.067.859023
TAGAGGG51200.067.3769762
GTAGGGC14000.067.140943
AAGGGCA48050.066.903394
Produced by FastQC (version 0.11.5)