FastQCFastQC Report
Mon 27 Feb 2023
SRR3126512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694918
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351561.3045294884668106No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38630.1433438791087521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117800.087.025411
ATAGGGC14950.077.329353
TAGGGCA13450.073.374894
AGGGATG35650.071.316195
CGTAGGG7300.070.835222
AGTAGGG43600.070.621062
ACGGGAT6900.070.1517644
ATAGGGA22450.069.288653
ATAGAGG19600.068.410531
AGGGAAT24900.067.5669255
TAGAGGG50250.067.449482
GTAGGGC14200.066.189963
ATAGCGG7350.065.930151
TATAGGG20550.065.880982
AGAGGGC39200.065.8167953
AGGGACT35350.065.8061145
GAGGGAT30950.065.747274
AGATAGG15100.065.7415161
AATAGGG36600.065.3756562
AAGAGGG92700.065.36612
Produced by FastQC (version 0.11.5)