FastQCFastQC Report
Mon 27 Feb 2023
SRR3126511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739585
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354031.292276019908125No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37960.13856113243429205No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC28740.10490640005694292TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117100.087.73541
ACGGGAT7450.076.323124
ATAGGGC16700.075.9756243
TAGGGCA15750.072.502464
ATAGCGG6700.071.6150741
AGTAGGG43900.071.50022
AGGGATG36600.070.873695
GTAGGGC15550.070.110753
ATAGAGG20700.069.993791
CGTAGGG8850.069.554192
ATAGGGA21350.069.5530553
AGATAGG13850.068.9485861
TAGGGTA10850.067.565024
GAATAGG15750.066.9031451
GTAGGGA18600.066.446153
AGGGCAC22100.065.491585
TAGAGGG52400.065.371962
AATAGGG37750.065.348812
AGGGCAT22350.065.179525
AGGGAAT27750.065.027235
Produced by FastQC (version 0.11.5)