FastQCFastQC Report
Mon 27 Feb 2023
SRR3126510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3068221
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442011.440606788102943No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44010.14343816824146632No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC31740.10344756782513385TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142200.087.901371
ATAGCGG8450.074.045491
ATAGGGC17150.073.988513
AGGGATG39450.073.7408755
TAGGGCA14450.073.503044
AGTAGGG48600.070.494612
ACGGGAT7900.070.197174
AGGGAAT31100.068.605695
AGATAGG16050.068.294321
TAGAGGG57600.068.046882
CGTAGGG9150.067.7980962
GTAGGGC13800.067.429623
GTAGGGA21800.067.260913
ACGGGTA3850.067.1377264
ATAGGGA23800.066.74283
AGGGACT39800.066.2436755
AGGGCAT28900.066.022645
AAGAGGG101950.065.735052
GAGGGAT33500.065.514394
ATAGAGG22300.065.397411
Produced by FastQC (version 0.11.5)