FastQCFastQC Report
Mon 27 Feb 2023
SRR3126509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023360
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168300.831784754072434No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66720.32974853708682583No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGCCCAGA21120.10438083188359956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55350.085.0507661
CGTAGGG5750.077.6886142
AGGGATG45350.076.4775165
ATGCGGG10300.073.957022
AGGGAAT25500.072.980985
GGATGGC32900.072.707067
ATAGCGG4150.072.598511
CGCTACG12850.072.413043
TAAGGGA23650.072.132373
ACGGGAT7150.071.643234
CGAAGGG18950.071.46362
AGGGCAT16950.070.97825
AGTAGGG29300.070.613332
ACGGGTA2000.070.4934
GTACGGG5950.070.335522
ATAGGGA21250.070.327133
GTAGGGA18150.069.910413
TAGCGGG11850.069.838662
GAGGGAT34450.069.2993554
CTACGAC13350.068.996895
Produced by FastQC (version 0.11.5)