FastQCFastQC Report
Mon 27 Feb 2023
SRR3126508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087356
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168980.8095408737177558No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC69170.33137615241482526No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG21510.10304902469918883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61650.084.0355761
CGTAGGG6450.079.415122
AGGGATG46400.075.859625
ATAGCGG3750.075.288551
AGGGAAT26350.075.084165
CGAAGGG21450.072.297462
CTACGAC13650.071.954925
AGTAGGG35550.071.911072
GGATGGC35550.071.519647
GACCGAT1450.071.305388
ATAGGGA22350.071.069953
ATGCGGG12000.070.881642
AGAGGGC28050.070.701143
AGACGGG12850.069.484332
TAAGGGA22100.069.3221743
GTAGGGA19850.069.130413
TAGCGGG10200.069.107872
ACGACCA14350.068.44827
TCCGCTA14300.068.1150741
ACGGGAT7200.067.880914
Produced by FastQC (version 0.11.5)