FastQCFastQC Report
Mon 27 Feb 2023
SRR3126507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346564
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215200.91708557703945No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC80760.3441627843945445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71050.084.263621
AGGGATG52150.075.701515
TAAGGGA26650.073.8918153
ATGCGGG13400.072.952152
ACGGGAT8900.072.873334
AGGGAAT30200.072.831375
AGTAGGG38400.072.4552842
ATAGCGG4700.072.046091
AATGCGG4900.071.9848251
CGAAGGG24100.070.399522
CTACGAC16450.070.282755
ATAGGGA25500.070.2205663
AAGAGGG78650.069.974162
GGATGGC39050.069.923867
CGTAGGG7000.069.825622
AAGGGAC25150.069.702834
GAGGGAT37300.069.5520254
TAGGGCA14650.069.294014
TAGGGAT21550.068.915894
GTAGGGC11750.068.397123
Produced by FastQC (version 0.11.5)