FastQCFastQC Report
Mon 27 Feb 2023
SRR3126506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560984
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225530.8806380672429035No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41580.16235946807945695No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG26340.10285109161166177Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80700.086.040711
CGTAGGG6100.076.317482
ATAGCGG5400.074.970471
AGTAGGG44750.070.719752
TAGGGCA15350.070.111834
AGGGCAT24000.068.732185
ACGGGAT6300.068.629614
ATAGAGG17300.068.298811
CGAAGGG23300.068.0131152
AGGGATG33300.067.319115
AGAGGGC38200.066.803733
ATAGGGC18400.066.6633153
TAGAGGG47800.066.5024262
AGGGAAT27950.066.248895
ATGCGGG12250.066.02542
TAGCGGG13350.065.868682
AAGAGGG84600.065.866812
GAATAGG15850.065.636981
GCGAGGG25600.065.57632
TAAGGGA21050.065.415353
Produced by FastQC (version 0.11.5)