FastQCFastQC Report
Mon 27 Feb 2023
SRR3126505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602208
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226280.8695692273638388No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42270.16243897490131456No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG28320.10883065458256987Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC26160.10053001143644166TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80000.085.774471
ATAGCGG4250.074.144441
AGTAGGG47400.071.680672
ATGCGGG10650.071.483792
TAGGGCA15150.071.035154
ATAGGGC18050.070.5573963
GTAGGGC14350.070.082953
ACGGGAT6000.069.709064
AGGGATG34300.069.327715
TATAGGG18550.069.1609342
CGTAGGG6500.068.683492
TAGCGGG13600.067.72662
GAATAGG15700.067.7021
AGGGACT36950.067.1536945
ATAGAGG16750.066.546661
GTACGGG6250.066.16752
AAGAGGG88450.066.09442
GTAGGGT9050.065.94863
CGAAGGG25850.065.809732
GTAGGGA21000.065.792823
Produced by FastQC (version 0.11.5)