FastQCFastQC Report
Mon 27 Feb 2023
SRR3126504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2907356
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285420.981716721309671No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46450.15976715613774164No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG29870.10273939620741318Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC29550.10163873980345028TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99850.085.508671
ACGGGAT6950.074.387664
CGTAGGG7850.074.241212
TAGGGCA17600.073.703724
GAATAGG18000.070.300981
AGGGCAT23900.070.007585
ATAGCGG5950.069.574051
AGTAGGG50750.068.068212
ATAGGGC21200.068.060563
AGAGGGC42800.067.5342943
AGGGATG36450.067.436845
GTAGGGA22500.067.052583
TAGAGGG52600.067.0144652
AGGGTAC15600.066.8838655
AGGGAAT31200.066.281315
AAGAGGG104450.066.1457442
CGAAGGG26150.066.1407552
ATAGAGG18750.065.732721
TAAGAGG25150.065.652421
AGATAGG18300.065.54971
Produced by FastQC (version 0.11.5)